Influence of the growth rate on the macromolecular composition of Acinetobacter calcoaceticus in carbon-limited chemostat culture |
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Authors: | JC du Preez PM Lategan DF Toerien |
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Institution: | Department of Genetics, University of Liverpool, Liverpool L69 3BX, U.K. |
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Abstract: | Abstract Infectious phage particles can be formed in vitro when extracts of T1-infected cells are incubated with T1 DNA. The DNA packaging system is based on mixtures of complementing extracts from Escherichia coli sup0 cells infected with the amber mutants am 4 (gene 16) or am 10 (gene 13). Gene 16 mutants are defective in the formation of DNA-filled heads but make proheads; gene 13 mutants are defective in prohead formation. Three forms of DNA have been packaged: (1) endogenous concatemeric DNA present in mixtures of am 4 and am 10 mutant extracts; (2) concatemeric DNA; (3) virion DNA both when supplied exogenously to mixtures of am 4 · am 20 and am 10 · am 20 double mutant extracts ( am 20 inhibits T1 DNA synthesis). The reaction requires added ATP, Mg2+ and spermidine for optimum efficiency and produces about 1.5 × 103 pfu/ μ g and about 1 × 104 pfu/ μ g for exogenous concatemeric and virion DNA, respectively. |
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Keywords: | In vitro complementation concatemeric DNA virion DNA prohead DNA-filled capsid DNA headful |
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