MEASUREMENTS OF IN VIVO RATES OF PROTEIN SYNTHESIS IN BRAIN, SPINAL CORD, HEART AND LIVER OF YOUNG VERSUS ADULT RATS, INTACT VERSUS HYPOPHYSECTOMIZED RATS |
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| Authors: | M. E. A. Reith P. Schotman W. H. Gispen |
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| Affiliation: | Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology and Laboratory of Physiological Chemistry, Medical Faculty, Institute of Molecular Biology, Padualaan 8, Utrecht, The Netherlands |
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| Abstract: | Abstract— In vivo protein synthesis rates in rats were estimated by single i.p. injections of large quantities of [1-14C]valine. This method gives reliable estimates of the precursor specific activity and average protein synthesis rates. In the brain, spinal cord, heart and liver, the average rates for adults were 0.65, 0.42, 0.49 and 1.92% replacement of protein-bound amino acid per h. In the brain and liver of 10-day olds the average rates were estimated to be 1.46 and 3.12% per h respectively. Hypophysectomy decreased synthesis rates by 25% or more in all tissues studied. The disadvantages of the method are that applying large amounts of valine i.p. appeared to constitute a stress and that the valine solution required for injection was hypertonic, causing withdrawal of body fluids of the animal. |
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