Molecular cloning and biochemical characterization of a novel erythrose reductase from <Emphasis Type="Italic">Candida magnoliae</Emphasis> JH110 |
| |
Authors: | Dae-Hee Lee Ye-Ji Lee Yeon-Woo Ryu Jin-Ho Seo |
| |
Institution: | (1) Department of Agricultural Biotechnology, Seoul National University, 151-921 Seoul, Korea;(2) Department of Molecular Science and Technology, Ajou University, 442-749 Suwon, Korea;(3) Department of Bioengineering, University of California San Diego, 92093 La Jolla, CA, USA |
| |
Abstract: | Background Erythrose reductase (ER) catalyzes the final step of erythritol production, which is reducing erythrose to erythritol using
NAD(P)H as a cofactor. ER has gained interest because of its importance in the production of erythritol, which has extremely
low digestibility and approved safety for diabetics. Although ERs were purified and characterized from microbial sources,
the entire primary structure and the corresponding DNA for ER still remain unknown in most of erythritol-producing yeasts.
Candida magnoliae JH110 isolated from honeycombs produces a significant amount of erythritol, suggesting the presence of erythrose metabolizing
enzymes. Here we provide the genetic sequence and functional characteristics of a novel NADPH-dependent ER from C. magnoliae JH110. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|