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Host factor for coliphage Q beta RNA replication: presence in procaryotes and association with the 30S ribosomal subunit in Escherichia coli.
Authors:Michael S DuBow  Thomas Ryan  Richard A Young and Thomas Blumenthal
Institution:(1) Department of Microbiology, Indiana University, 47401 Bloomington, Indiana, USA;(2) Present address: Cold Spring Harbor Laboratory, 11724 Cold Spring Harbor, N.Y., USA;(3) Present address: Department of Molecular Biophysics and Biochemistry, Yale University, 06520 New Haven, Connecticut, USA
Abstract:Summary The Host Factor required for in vitro coliphage Qbeta RNA replication, a heat-stable RNA binding protein present in uninfectedEscherichia coli, has been detected by both immunological and functional tests inAcinetobacter calcoaceticus, Klebsiella pneumoniae, Pseudomonas aeruginosa andPseudomonas putida. It was not detectable by these criteria inBacillus stearothermophilus, Bacillus subtilis, Caulobacter crescentus, Micrococcus lysodeikticus, Rhodopseudomonas capsulata orSaccharomyces cerevisiae. InEscherichia coli the Host Factor protein has been shown to be associated with ribosomes. It is demostrated here that this association is specific for the 30S ribosomal subunit.
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