TRAIL在细胞中的表达及其诱导凋亡的活性分析

以PCR方法克隆了Trail cDNA全长，构建了其真核表达载体，通过脂质转染HeLa细胞，48小时后利用流式细胞仪分析Trail诱导细胞凋亡的比率，发现发生凋亡的细胞为总细胞数的19％。证实了Trail真核4表达系统的产物的生物学活性高，为从真核表达的途径获得Trail基因工程产品奠定了基础。

Expression of Trial inMammalian Cell and Its Apoptosis Activation in Culture Cells

To develop a system for expression of Trail in mammalian cell. PCR and sequencing were used to clone and to confirm Trail gene. Then, we constructed the vector for expression of Trail in mammalian cells. HeLa cells, one of sensitive cell lines to Trail, were transfected with the vector DNA. After 48h, flow cytometry was used to screen HeLa transfected by Trail. 19% of total cells happened to apoptosis . This study conforms that the production of Trail expressing in mammal cell can induce apoptosis and exhibit high bio-active. The results indicate the potential application of Trail to tumor immunotherapy.