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Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
Authors:Sebastián F. Cavalitto  Roque A. Hours  Carlos F. Mignone
Affiliation:(1) Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI), CONICET-UNLP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, 1900 La Plata, Argentina
Abstract:Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1–1, reaction time of 10 min and up to 0.17 PGase units per test tube.
Keywords:endopolygalacturonases  Geotrichum klebahnii  pectin  pectin-releasing enzymes  protopectinases
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