Relationship between oxidative stress and mitochondrial function in the post-conditioned heart |
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Authors: | Francisco Correa Noemí García Cinthya Robles Eduardo Martínez-Abundis Cecilia Zazueta |
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Affiliation: | (1) Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano No. 1., Col. Sección XVI, Mexico, D.F., 14080, Mexico |
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Abstract: | The pathways activated by post-conditioning may converge on the mitochondria, in particular on the mitochondrial permeability transition pore. We sought to characterize the inhibition status of the mitochondrial permeability transition early after the post-conditioning maneuver and before long reperfusion was established. We observed that post-conditioning maneuvers applied to isolated rat hearts, after a prolonged ischemia and before reperfusion, promoted cardiac mechanical function recovery and maintained mitochondrial integrity. These effects were evaluated by mitochondrial swelling, calcium transport, and NAD+ content measurements; the improvements were established before restoring a long lasting reperfusion period. Mitochondrial integrity was associated with a diminution in oxidative stress, since carbonylation of proteins was prevented and aconitase activity was preserved in the post-conditioned hearts, implying that ROS might mediate mitochondrial dysfunction and mPTP opening. In addition, we found that cytochrome release was significantly abolished in the post-conditioned heart, in contrast with conventionally reperfused hearts. |
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Keywords: | Post-conditioning Mitochondria Permeability transition pore Ischemic-reperfused heart CSA, cyclosporin A |
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