Isolation via enrichment and characterization of 14 dinucleotide microsatellite loci in one catfish, Glyptosternum maculatum and cross-amplification in other related taxa |
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| Authors: | Baoying Guo Congxin Xie Hongjing Li Lei Shan Huanling Wang Bin Huo Yan Zhang Qing Zhou |
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| Affiliation: | (1) Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, P.R. China;(2) Key Laboratory of Freshwater Fish Germplasm Resources & Biotechnology, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou, 434000, China |
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| Abstract: | A total of 14 novel dinucleotide microsatellite loci were developed using genomic DNA enrichment protocol and characterized for one Glyptosternoid catfish, Glyptosternum maculatum. Polymorphism was explored using 36 individuals from one natural population. The observed number of alleles ranged from 2 to 9. The ranges of observed and expected heterozygosity were 0.0278 to 1 and 0.4319 to 0.8498, respectively and the average of PIC was 0.5754. In addition, successful cross-species amplification of these set of microsatellite markers in the related taxa, Pseudechenneis sulcatus (McClelland) suggested that they would provide a useful tool for the genetic and conservation studies of Sisoridae species. |
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| Keywords: | Glyptosternum maculatum Genetic diversity Microsatellites Cross-species amplification |
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