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Strategies for spectral assignment in the 1H NMR spectra of a 25 000 Mr murine antibody fragment: (i) in vivo deuteration and (ii) use of a denaturant
Authors:Peter Gettins  Raymond A. Dwek
Affiliation:Department of Biochemistry, South Parks Road, Oxford, OX1 3QU, England
Abstract:
Keywords:DNP, 2,4-dinitrophenyl  Fv fragment, variable region of light and heavy chains  NMR, nuclear magnetic resonance  ppm, parts per million  TNP, 2,4,6-trinitrophenyl
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