Plasmodium falciparum: Assay in vitro for inhibitors of merozoite penetration of erythrocytes |
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Authors: | Martin M. Weiss Joel D. Oppenheim Jerome P. Vanderberg |
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Affiliation: | 1. Division of Parasilology, New York University Medical Center, 550 First Avenue, New York, New York 10016, U.S.A.;2. Department of Microbiology, New York University Medical Center, 550 First Avenue, New York, New York 10016, U.S.A. |
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Abstract: | Highly synchronous cultures of the erythrocytic stages of Plasmodium falciparum were used both to assay penetration of merozoites into human red blood cells, and to subsequently study the inhibitory effects of various substances on penetration. While several sugars exhibited no inhibitory effect, fucose, glucosamine-HCl, and N-acetyl glucosamine, when added to synchronous cultures at the schizont stage, inhibited invasion. On further testing fucose and glucosamine-HCl were found to be toxic to the intracellular growth and development of the parasite; only N-acetyl glucosamine had an inhibitory effect solely related to the inhibition of merozoite penetration. Glycophorin A, the major glycoprotein of the red blood cell surface, had no inhibitory effect at low concentrations, but had a slight effect at higher (500 μg/ml) levels. |
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Keywords: | Malaria Malaria Protozoa, parasitic Merozoite penetration Sugar inhibition Fucose Lactose Galactose Mannose Glucosamine-HCl Glycoprotein Glycophorin A Erythrocytes, human |
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