An improved treatment of data for estimation of molecular weights by sephadex gel filtration |
| |
Authors: | A K Grover M Kapoor |
| |
Institution: | Department of Biology, The University, Calgary, Alberta, Canada |
| |
Abstract: | A method for analysis of elution data of proteins, obtained from Sephadex gel filtration experiments, is described. The relevant elution data from seven different proteins, with known molecular weights and Stoke's radii, were fitted into various equations relating elution parameters and molecular size parameters. It was observed that polynomial relationships represented elution data for proteins with a much greater degree of precision than linear equations. The validity of this procedure was also checked by analysing gel filtration data available in the literature and it was concluded that a better fit was obtained using polynomial relationships, provided a sufficiently large number of experimental points were available for numerical analysis. Using this method, values of 320,000 ± 7000 for the molecular weight, and (60 ± 0.4) × 10?8 cm for the Stoke's radius of Neurospora NAD-specific glutamate dehydrogenase were calculated. |
| |
Keywords: | To whom reprint requests should be sent |
本文献已被 ScienceDirect 等数据库收录! |
|