Involvement of the cell wall ofNocardia restricta on the bioconversions of steroid sapogenins |
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Authors: | T. Kastelic-Suhadolc I. Belič |
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Affiliation: | (1) Institute of Biochemistry, Medical Faculty, University E. Kardelj, 61000 Ljubljana, Yugoslavia |
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Abstract: | The bioconversion of tigogenin, (25R)-5-spirostan-3-ol byNocardia restricta to (25R)-4-spirosten-3-one and (25R)-1,4-spirostadien-3-one was strongly increased using protoplasts instead of intact cells. The same effect could be achieved by adding-cyclodextrin to the medium. Since 5-steroid sapogenins pass through the cell wall without hindrance, it can be concluded that the cell wall ofNocardia restricta is a rate-limiting factor only for 5-isomers. |
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