| 盐酸己烷基双胍对肌酸激酶的活力及构象的影响 |
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| 引用本文： | 李学刚,邹承鲁. 盐酸己烷基双胍对肌酸激酶的活力及构象的影响[J]. 生物物理学报, 1993, 9(1): 1-5 |
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| 作者姓名： | 李学刚 邹承鲁 |
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| 作者单位： | 生物大分子国家重点实验室生物物理研究所，生物大分子国家重点实验室生物物理研究所 北京100101，北京100101 |
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| 摘 要： | 测定了盐酸己烷基双胍(C_6H_(13)C_2N_5H_(?)HCl;记为HBGC)对肌酸激酶(Creatine Kinase;记为CK)的活力的影响;同时利用荧光光谱,紫外差光谱.付里叶红外光谱等手段测定了HBGC对CK构象的影响,结果表明.HBGC为0.07mol时就使CK完全失活、而且在高浓度的HBGC中变性后的CK.稀释时即可完全复活;CK的活力丧失明显先于构象的变化,与溴化+烷基三甲铵不同,HBGC可以破坏CK的二级结构,使之完全无序化.
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| 关 键 词： | 盐酸己烷基 双胍 肌酸激酶 |
THE EFFECT OF HEXVXBIGUANIDINE HYDROCHLORIDE ON THE ACTIVITY AND CONFORMATION OF CREATINE KINASE |
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| Li Xuegang Chenlu Tsou. THE EFFECT OF HEXVXBIGUANIDINE HYDROCHLORIDE ON THE ACTIVITY AND CONFORMATION OF CREATINE KINASE[J]. Acta Biophysica Sinica, 1993, 9(1): 1-5 |
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| Authors: | Li Xuegang Chenlu Tsou |
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| Abstract: | With fluorescence, ultraviolet difference and Fourier transform infrared spectroscopy (FTIR) techniques, the effect of hexylbiguanidine hydrochloride (HBGC) on the activity and conformation of creatine kinase (CK) were studied. At a HBGC concentration of 0.07mol'L. C K would be entirely inactivated. After C K is denatured at a high concentration of HBGC, it is very easy to be renatured by dilution. The change of activity is much earlier than that of conformation. It is different from Decyltrimethyl Ammonium Bromide that HBGC can cause the secondary structure of C K to be randomized. |
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| Keywords: | Hexylbiguanidine hydrochloride Great |
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