Larvicidal toxin from Bacillus sphaericus spores: Isolation of toxic components |
| |
Authors: | Regina Tinelli Catherine Bourgouin |
| |
Affiliation: | Institut Pasteur, 75724 Paris Cedex 15, France |
| |
Abstract: | Meiotic maturation of amphibian oocytes induced by progesterone is known to be regulated by protein phosphorylation. To investigate a possible role for protein phosphatase-1 in this process, the effect of phosphatase inhibitor-2 was determined on the in vivo rate of dephosphorylation of phosphorylase a and on the rate of oocyte maturation. Dephosphorylation of microinjected phosphorylase a was inhibited up to 40% in the presence of inhibitor-2, with half-maximal inhibition at an intracellular concentration of 0.6 μM. Inhibitor-2 also caused over a 3-fold increase in the half-time for maturation, suggesting a possible role for protein phosphatase-1 in the regulation of meiosis. |
| |
Keywords: | Membrane potential Luminescence Recombination kinetics Reaction center carotenoid Triplet yield |
本文献已被 ScienceDirect 等数据库收录! |