Immature embryos culture in Italian red chicory |
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Authors: | Varotto Serena Lucchin Margherita Parrini Paolo |
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Institution: | (1) Department of Agronomy and Crop Production, University of Padova, Agripolis, Via Romea, 16, 35020 Legnaro, Padova, Italy |
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Abstract: | Chicory (Cichorium intybus L.) embryo maturation in vitro was achieved in B5 medium without growth regulators. Immature embryos were collected at different developmental stages 4–168
h after pollination. Only a few plantlets were obtained from ovules collected shortly after pollination since the embryos
at these early stages developed abnormal leaves and roots which regenerated shoots ‘via’ organogenesis. Zygotic embryos collected
24–72 h after pollination needed one month of culture in vitro before acclimation. Embryos collected at the heart or early torpedo stage of development (three days after pollination) produced
green plantlets after two weeks of in vitro culture and thus they could be transferred to the greenhouse for acclimation. Culture of zygotic embryos in vitro has shown to be a suitable technique to accelerate the breeding process in biennial cultivated Italian red chicory. In fact
it is possible to obtain plantlets ready for performing the selection for the desired agronomic traits one month after pollination.
Since flowering occurs after vernalization, the selection can be performed in the correct season before flowering.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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