Terbium identifies double-stranded RNA on gels by quenching the fluorescence of intercalated ethidium bromide |
| |
Authors: | M Al-Hakeem S S Sommer |
| |
Institution: | Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, Minnesota 55905. |
| |
Abstract: | We report that the lanthanide cation terbium quenches the fluorescence of ethidium bromide bound to double-stranded RNA by 40-fold, whereas the quenching of double-stranded and single-stranded DNA is under 2.5-fold and the quenching of single-stranded RNA is under 5-fold. This observation was used to develop a convenient method of detecting dsRNA among other nucleic acids in an agarose or polyacrylamide gel. The sensitivity of the method is approximately 4 ng/mm2. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|