Bacterial community composition of a wastewater treatment system reliant on N2 fixation |
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Authors: | Nicola M Reid Tracey H Bowers Gareth Lloyd-Jones |
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Institution: | (1) Scion, Private Bag 3020, Rotorua Mail Centre, Rotorua, 3046, New Zealand |
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Abstract: | The temporal stability and change of the dominant phylogenetic groups of the domain bacteria were studied in a model plant-based
industrial wastewater treatment system showing high levels of organic carbon removal supported by high levels of N2 fixation. Community profiles were obtained through terminal restriction fragment length polymorphism analysis and cloning
of 16S rRNA amplicons followed by sequencing. Bacterial community profiles showed that ten common terminal restriction fragments
made up approximately 50% of the measured bacterial community. As much as 42% of the measured bacterial community could be
monitored by using quantitative PCR and primers that targeted three dominant operational taxonomic units. Despite changes
in wastewater composition and dissolved oxygen levels, the bacterial community composition appeared stable and was dominated
by α-Proteobacteria and β-Proteobacteria, with a lesser amount of the highly diverse bacterial phylum Bacteroidetes. A short
period of considerable change in the bacterial community composition did not appear to affect treatment performance indicating
functional redundancy in this treatment system. |
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Keywords: | Biorefinery Wastewater Nitrogen fixation T-RFLP qPCR 16S |
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