Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans |
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| Authors: | M. Denijn R. A. De Weger A. D. M. Van Mansfeld J. A. M. van Unnik C. J. M. Lips |
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| Affiliation: | (1) Division of Histochemistry, Cytochemistry and Electron Microscopy, Department of Pathology, University Hospital Utrecht, P.O. Box 85500, NL-3508 GA Utrecht, The Netherlands;(2) Institute of Molecular Biology and Medical Biology and Laboratory of Physiological Chemistry, University of Utrecht, Padualaan 8, NL-3584 CH Utrecht, The Netherlands;(3) Department of Pathology, University Hospital Utrecht, P.O. Box 85500, NL-3508 GA Utrecht, The Netherlands;(4) Division of Internal Medicine and Dermatology, University Hospital Utrecht, P.O. Box 85500, NL-3508 GA Utrecht, The Netherlands;(5) Present address: Division of Pathology, University Hospital Nijmegen, Geert Grooteplein Zuid 24, NL-6525 GA Nijmegen, The Netherlands |
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| Abstract: | Summary We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A 35S-labeled, IAPP-specific DNA probe — hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.Abbreviations DNA Deoxyribonucleic acid - dpm Disintegration per minute - dCTP Deoxycytidine triphosphate - EDTA Ethylene diamine tetraacetic acid - IAPP Islet amyloid polypeptide - PBS Phosphate buffered saline - RNA Ribonucleic acid - SSC Standard sodium citrate |
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