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An IS15 insertion generates an eight-base-pair duplication of the target DNA |
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| Authors: | Patrick Trieu-Cuot Agnès Labigne-Roussel Patrice Courvalin |
| Institution: | Laboratoire de Biochimie L.A. CNRS 271, Unité de Bactériologie Médicale, Institut Pasteur, 75724 ParisFrance Tel. (1) 306.19.19 Ext. 3516 |
| Abstract: | In plasmid pIP1088 the transposable module IS15 is inserted at nucleotide position 1,430 of the vector plasmid pBR322. We have sequenced the termini of the IS15 element, which consists of two perfect inverted repeat sequences, 14 bp long. The sequence is 5′-GGCACTGTTGCAAA… TTTGCAACAGTGCC-3′. The integration event results in the duplication of 8 bp of target DNA. |
| Keywords: | Insertion elements transposons inverted repeated sequences chemical and dideoxy sequencing pBR322 plasmid kanamycin resistance Ap ampicillin bp base pairs IS insertion sequence (Fiandt et al 1972) Km kanamycin L left p plasmid R right RF double-stranded replicative form of DNA Tc tetracycline Tn transposon (see also legends to Figs 1–3) |
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