Identification of a major quantitative trait locus (QTL) for yellow spot (<Emphasis Type="Italic">Mycovellosiella koepkei</Emphasis>) disease resistance in sugarcane |
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Authors: | S M Aljanabi Y Parmessur H Kross S Dhayan S Saumtally K Ramdoyal L J C Autrey A Dookun-Saumtally |
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Institution: | (1) Biotechnology Department, Mauritius Sugar Industry Research Institute, Réduit, Mauritius;(2) Zoology and Genetic Department, Iowa State University, Ames, 50011 Iowa, USA |
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Abstract: | In this study we used amplified fragment length polymorphism (AFLP) and microsatellite (short sequence repeat or SSR) markers
to identify a major quantitative trail locus (QTL) for yellow spot (Mycovellosiella koepkei) disease resistance in sugarcane. A bi-parental cross between a resistant variety, M 134/75, and a susceptible parent, R 570,
generated a segregating population of 227 individuals. These clones were evaluated for yellow spot infection in replicated
field trials in two locations across two consecutive years. A χ2-test (χ2 at 98% confidence level) of the observed segregation pattern for yellow spot infection indicated a putative monogenic dominant
inheritance for the trait with a 3 (resistant):1(susceptible) ratio. The AFLP and SSR markers identified 666 polymorphisms
as being present in the resistant parent and absent in the susceptible one. A genetic map of M 134/75 was constructed using
557 single-dose polymorphisms, resulting in 95 linkage groups containing at least two markers based on linkages in coupling.
QTL analysis using QTLCartographer v1.17d and MAPMAKER/QTL v1.1 identified a single major QTL located on LG87, flanked by an AFLP marker, actctc10, and an SSR marker, CIR12284. This
major QTL, which was found to be linked at 14 cM to an AFLP marker, was detected with LOD 8.7, had an additive effect of −10.05%
and explained 23.8% of the phenotypic variation of yellow spot resistance. |
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Keywords: | Linkage mapping Molecular markers Mycovellosiella koepkei Polyploidy Quantitative resistance Sugarcane |
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