Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI‐10 and extracellular matrix protein tenascin‐C on human glioblastoma cell |
| |
| Authors: | Yongjun Li Haiyan Qiao Wei Yan Jing Zhang Chunyan Xing Hongda Wang Bailin Zhang Jilin Tang |
| |
| Institution: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, , Changchun, 130022 China;2. Graduate University of Chinese Academy of Sciences, , Beijing, 100049 China;3. The Affiliated Hospital to Changchun University of Chinese Medicine, , Changchun, Jilin, China;4. +86 431 85262430;5. +86 431 85262734 |
| |
| Abstract: | Molecular recognition force spectroscopy (MR‐FS) was applied to investigate the dynamic interaction between aptamer GBI‐10 and tenascin‐C (TN‐C) on human glioblastoma cell surface at single‐molecule level. The unbinding force between aptamer GBI‐10 and TN‐C was 39 pN at the loading rate of 0.3 nN sec?1. A series of kinetic parameters concerning interaction process such as the unbinding force fu, the association rate constant kon, dissociation rate constant at zero force koff, and dissociation constant KD for aptamer GBI‐10/TN‐C complexes were acquired. In addition, the interaction of aptamer GBI‐10 with TN‐C depended on the presence of Mg2+. This work demonstrates that MR‐FS can be used as an attractive tool for exploring the interaction forces and dynamic process of aptamer and ligand at the single‐molecule level. As a future perspective, MR‐FS may be used as a potential diagnostic and therapeutic tool by combining with other techniques. Copyright © 2012 John Wiley & Sons, Ltd. |
| |
| Keywords: | aptamer molecular recognition force spectroscopy tumor‐targeting biomarker |
|
|
