Molecular arrangement between multivalent glycocluster and Pseudomonas aeruginosa LecA (PA‐IL) by atomic force microscopy: influence of the glycocluster concentration |
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Authors: | D. Sicard Y. Chevolot E. Souteyrand A. Imberty S. Vidal M. Phaner‐Goutorbe |
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Affiliation: | 1. Université de Lyon, Ecole Centrale de lyon, Institut des Nanotechnologies de Lyon (INL, UMR CNRS 5270), , 69134 Ecully, France;2. Laboratoire Interdisciplinaire Carnot de Bourgogne (ICB UMR CNRS 6303) – Dept Nanosciences/OSNC, Faculty of Sciences Mirande, Université de Bourgogne, , 21078 Dijon, France;3. CERMAV (CNRS, UPR 5301), Université Joseph Fourier, , 38041 Grenoble, France;4. Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR CNRS 5246), Laboratoire de Chimie Organique 2‐Glycochimie, Université de Lyon, , 69622 Villeurbanne, France |
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Abstract: | New therapeutics strategy against cystic fibrosis seeks to prevent the adhesion of the bacterium Pseudomonas aeruginosa (PA) on the epithelial cells in the lungs. One of the factors that induces the adhesion is the interaction between natural glycocluster present on the cells and lectins such as the PA lectin LecA (PA‐IL) present on the bacterium. By introducing synthetic glycoclusters with a great affinity with the lectin PA‐IL, the adhesion can be prevented. In this study, we characterized, by atomic force microscopy, the interaction between a tetra‐galactosylated glycocluster and the PA‐IL lectin for high concentration of lectins (2.5 μM).We showed that the strong lectin/lectin interaction is reduced even for low concentration of glycoclusters (1 for 20 000 lectins). We assumed that it is due to the tensioactive behavior of the glycoclusters. It was shown that the arrangement of the created complexes induced different structures evolving from one‐dimensional elongated aggregates to two‐dimensional compact islands when increasing the glycocluster concentration. This evolution can be interpreted as the predominance of the glycocluster/lectin interaction. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | AFM protein glycocluster inter molecular interaction lectin Pseudomonas aeruginosa cystic fibrosis |
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