An improved purification method for cytosolic malate dehydrogenase from several sources |
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| Authors: | C Domènech J J Abante F X Bozal A Mazo A Cortés J Bozal |
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| Affiliation: | Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain. |
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| Abstract: | A new purification method for cytosolic malate dehydrogenases from several sources has been developed. The procedure, employing chromatographies on 5'AMP-Sepharose, DEAE-Sephacel and Blue-Sepharose, allows for a rapid isolation of the enzyme (approximately 40 hours), in large quantities, with good yields (45-54%). The specific activity of final preparations were around 1300 I.U./mg and were judged homogeneous by polyacrylamide gradient gel and sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance size exclusion chromatography and isoelectric focusing. |
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