Adhesion of Salmonella dublin to HEp2 epithelial cells

Two strains of Salmonella dublin , grown serially in brain heart infusion broth, were motile and produced mannose sensitive (MS) but not mannose resistant (MR) haemagglutinins; grown on phosphate buffered agar, the strains were poorly motile and phenotypically MSHA^- MRHA ⁺. In adhesion tests with HEp2 epithelial cells, broth grown bacteria that were motile and MSHA⁺ MRHA^- adhered better than agar grown bacteria that were poorly motile and MSHA^- MRHA⁺. Thus, in adhesion tests with HEp2 epithelial cells, strains of S. dublin behaved like S. typhimurium strains in that their HEp2 cell adhesiveness was associated with motility and production of MSHA.