Detection of Enterobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture |
| |
| Authors: | Mullane N R Murray J Drudy D Prentice N Whyte P Wall P G Parton A Fanning S |
| |
| Institution: | Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. |
| |
| Abstract: | Enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. Contaminated infant milk formula (IMF) has been implicated in cases of E. sakazakii meningitis. Quick and sensitive methods to detect low-level contamination sporadically present in IMF preparations would positively contribute towards risk reduction across the infant formula food chain. Here we report on the development of a simple method, combining charged separation and growth on selective agar, to detect E. sakazakii in IMF. This protocol can reliably detect 1 to 5 CFU of E. sakazakii in 500 g of IMF in less than 24 h. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
