首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Cytoskeletal changes and induction of embryogenesis in microspore and pollen cultures of Brassica napus L.
Authors:B Hause  G Hause  P Pechan  A A M van Lammeren
Institution:1. Department of Botany and Plant Sciences and Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, USA;2. Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, China;3. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai 200032, People’s Republic of China;4. FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;5. Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;1. MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, 73000, China;2. College of Life Sciences, Northwest Normal University, Lanzhou, Gansu, 730070, China;3. Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, Qinghai, 810016, China;4. College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China
Abstract:Microspores and pollen of Brassica napus were cultured under conditions leading to embryo formation. Concomitant changes in cytoskeletal configurations were analysed. The microfilamental cytoskeleton exhibited a loss of polarity in embryogenic cells but cytochalasin treatment revealed that microfilaments do not influence embryogenesis. Two embryogenic pathways started from microspores and were either characterized by turned division planes or by division when the nucleus was in the cell centre. In both cases microtubules clearly exhibited new arrangements and likely played a major role in newly induced symmetrical division. In pollen, embryogenic development started in the vegetative cell provided the generative cell was arrested near the pollen wall. The concomitant disappearance of defined microtubular arrays is likely to be responsible for the positioning of the cell.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号