A comparison of bovine serum albumin and chicken ovalbumin as supplements for the serum-free growth of chinook salmon embryo cells,CHSE-214. |
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| Authors: | A. Barlian R. C. Ganassin D. Tom N. C. Bols |
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| Affiliation: | 1. Geisinger Commonwealth School of Medicine, Scranton, PA;2. Children''s Hospital of Pittsburgh of UPMC, Pittsburgh, PA;3. University of Pittsburgh School of Medicine, Pittsburgh, PA;1. Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia;2. Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia;3. Centre for High-Throughput Technologies, Department of Biotechnology, University of Rijeka, Rijeka, Croatia;4. Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia;5. Medical Faculty, University of Belgrade, Belgrade, Serbia;6. University Children''s Hospital of Belgrade, Belgrade, Serbia |
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| Abstract: | Chicken ovalbumin and bovine serum albumin (BSA) were compared as supplements to the basal medium, L-15, for the serum-free cultivation of the Chinook Salmon Embryo cell line, CHSE-214. Unlike L-15 alone, ovalbumin and some commercial BSA preparations allowed cell proliferation and development of confluent monolayer cultures. However, only a fatty acid-free BSA (2%) supported continuous proliferation for two years through approximately 15 subcultivations. For this, subcultivation was achieved with non enzymatic cell dissociation solutions. Also, new serum-free subcultivation techniques were developed that utilized avian egg white trypsin inhibitors to terminate the action of either bovine or cod trypsin. Finally, CHSE-214 were successfully cryopreserved in 2% BSA, allowing all cell cultivation steps to be performed in the absence of FBS. |
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