Expression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens |
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Authors: | Maria Jos Carmona Antonio Molina Jos Antonio Fernndez Juan Jos Lpez-Fando Francisco García-Olmedo |
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Institution: | Maria José Carmona,Antonio Molina,José Antonio Fernández,Juan José López-Fando,Francisco García-Olmedo |
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Abstract: | Thionins are cysteine-rich, 5 kDa polypeptides which are toxic to plant pathogens in vitro. Expression of the gene encoding α-thionin from barley endosperm, under the 35S promoter from cauliflower mosaic virus, conferred to transgenic tobacco enhanced resistance to the bacterial plant pathogens Pseudomonas syringae pv. tabaci 153 and P. syringae pv. syringae. The barley α-thionin gene, which has two introns, was correctly spliced in tobacco. The α-thionin in transgenic plants had the expected mobility in the gradient, when separated by high-performance liquid chromatography, reacted with monospecific antibodies and showed the expected antibiotic properties in vitro. |
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