A colorimetric method for α-naphthol and its application to assay of hydrolases |
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| Authors: | Bernard Weissmann |
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| Institution: | Department of Biological Chemistry, University of Illinois College of Medicine, Chicago, Illinois 60612 USA |
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| Abstract: | Coupling of α-naphthol with diazotized Aniline Yellow in sodium carbonate solution produces a water-soluble dye (λmax 580 mμ). The reaction serves as the basis of a sensitive estimation of α-naphthol. The general applicability of this colorimetry to assay of hydrolases, using naphthyl esters and glycosides as substrates, is illustrated by an assay of β-acetylglucosaminidase. |
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