Voltage‐gated K+ channels play a role in cAMP‐stimulated neuritogenesis in mouse neuroblastoma N2A cells |
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| Authors: | Yuk‐Man Leung Chien‐Fang Huang Chia‐Chia Chao Dah‐Yuu Lu Chang‐Shin Kuo Tzu‐Hurng Cheng Li‐Yun Chang Chun‐Hsiao Chou |
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| Institution: | 1. Graduate Institute of Neural and Cognitive Sciences, China Medical University, Taichung, Taiwan;2. Department of Biological Sciences and Technology, China Medical University, Taichung, Taiwan;3. Graduate Institute of Biosystems Molecular Medicine, China Medical University, Taichung, Taiwan |
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| Abstract: | Neuritogenesis is essential in establishing the neuronal circuitry. An important intracellular signal causing neuritogenesis is cAMP. In this report, we showed that an increase in intracellular cAMP stimulated neuritogenesis in neuroblastoma N2A cells via a PKA‐dependent pathway. Two voltage‐gated K+ (Kv) channel blockers, 4‐aminopyridine (4‐AP) and tetraethylammonium (TEA), inhibited cAMP‐stimulated neuritogenesis in N2A cells in a concentration‐dependent manner that remarkably matched their ability to inhibit Kv currents in these cells. Consistently, siRNA knock down of Kv1.1, Kv1.4, and Kv2.1 expression reduced Kv currents and inhibited cAMP‐stimulated neuritogenesis. Kv1.1, Kv1.4, and Kv2.1 channels were expressed in the cell bodies and neurites as shown by immunohistochemistry. Microfluorimetric imaging of intracellular K+] demonstrated that K+] in neurites was lower than that in the cell body. We also showed that cAMP‐stimulated neuritogenesis may not involve voltage‐gated Ca2+ or Na+ channels. Taken together, the results suggest a role of Kv channels and enhanced K+ efflux in cAMP/PKA‐stimulated neuritogenesis in N2A cells. J. Cell. Physiol. 226: 1090–1098, 2011. © 2010 Wiley‐Liss, Inc. |
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