Quantitation of oxypurines and allopurinol metabolites in biological fluids by cation-exchange chromatography |
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| Authors: | Lawrence Sweetman William L Nyhan |
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| Institution: | Department of Pediatrics, University of Miami School of Medicine, Miami, Florida 33152 USA |
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| Abstract: | Chromatography of urine, cerebrospinal fluid, and deproteinized serum on 30 × 0.9 cm columns of AG 50 permits the convenient separation of hypoxanthine, xanthine, allopurinol, and its metabolites. Quantitation by determination of absorbance at 250 mμ is accurate and can be automated. Column preparation is simple, and the columns can be regenerated and used daily for several months with no loss of resolution. |
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