|
|||||
|
|
FISH-aimed karyotype analysis in <Emphasis Type="Italic">Aconitum</Emphasis> subgen. <Emphasis Type="Italic">Aconitum</Emphasis> reveals excessive rDNA sites in tetraploid taxa |
|
| Authors: | |
| Institution: | 1.Department of Plant Cytology and Embryology, Institute of Botany,Jagiellonian University,Kraków,Poland;2.Department of Plant Anatomy and Cytology,University of Silesia in Katowice,Katowice,Poland;3.Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology,University of Technology and Life Sciences in Bydgoszcz,Bydgoszcz,Poland;4.Department of Plant Breeding and Seed Science,University of Agriculture in Kraków,Kraków,Poland |
| Abstract: | The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2n?=?2x?=?16; Aconitum variegatum, A. degenii) and tetraploids (2n?=?4×?=?32; A. firmum, A. plicatum), rDNA repeats were localized exclusively on the shorter arms of chromosomes, in subterminal or pericentromeric sites. All analyzed species showed similar basal genome size (Cx?=?5.31–5.71 pg). The most striking features of tetraploid karyotypes were the conservation of diploid rDNA loci and emergence of many additional 5S rDNA clusters. Chromosomal distribution of excessive ribosomal sites suggests their role in the secondary diploidization of tetraploid karyotypes. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |