Phosphorylation of Extracellular Signal-Regulated Kinases 1/2 Predominantly Enhanced in the Microglia of the Rat Spinal Cord Following Lipopolysaccharide Injection |
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Authors: | Dan Zhou Min Fei Qin Shen Chun Cheng Youhua Wang Jian Zhao Hai-Ou Liu Linlin Sun Yonghua Liu Xiaowei Yu Aiguo Shen |
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Institution: | (1) The Jiangsu Province Key Lab of Neuroregeneration, Nantong University, Nantong, 226001, People’s Republic of China;(2) Department of Biochemistry, Medical College of Nantong University, Nantong, 226001, People’s Republic of China;(3) Institute of Medical Biotechnology, Soochow University, Suzhou, 215007, People’s Republic of China;(4) Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, People’s Republic of China |
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Abstract: | The present study was initiated to investigate the role of extracellular signal-regulated kinases (ERK) 1/2 signaling pathway
in the early response of spinal cord to systemic inflammation by using Western blotting and immunohistochemical techniques
in a rat model intraperitoneally injected with 10 mg/kg of lipopolysaccharide (LPS). The results showed that there was a considerable
amount of phosphorylated ERK 1/2 protein in the spinal cord of inflamed animals killed under pentobarbital anesthesia. The
result of Western blotting showed that the phosphorylation level of ERK 1/2 in the spinal cord was increased at one hour;
then 12 and 24 h after LPS injection the level decreased, while the total ERK 1/2 level seemed unchanged. The phosphorylated
ERK 1/2 dominantly existed in the microglia cells of the gray matter of spinal cord, as demonstrated with double immunofluorescent
staining 1 h after LPS injection. Collectively, the present results suggest that ERK signal pathway involve the cellular activation
in the spinal cord following systemic inflammation, with ERK mainly in microglia. The increase of phosphorylation of ERK 1/2
in microglia of spinal cord after LPS injection implicates that ERK signaling pathway involves intracellular activity of microglia
responding to the inflammation.
Dan Zhou and Min Fei contributed equally to this work. |
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Keywords: | ERK Microglia Lipopolysaccharide Spinal cord Rat |
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