Dominant bacterioplankton populations in the meromictic Lake Suigetsu as determined by denaturing gradient gel electrophoresis of 16S rRNA gene fragments |
| |
Authors: | Ryuji Kondo Akie Nakagawa Lisa Mochizuki Kyoko Osawa Yukiyasu Fujioka Junki Butani |
| |
Institution: | (1) Department of Marine Bioscience, Fukui Prefectural University, Obama Fukui, 917-0003, Japan |
| |
Abstract: | Lake Suigetsu is a typical meromictic lake in Japan characterized by a permanent chemocline at a depth of between 3 and 8 m
separating the oxic freshwater mixolimnion from anoxic saline sulfidogenic monimolimnion. Dominant bacterioplankton populations
in Lake Suigetsu were investigated using PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. The
bacterial population was vertically stratified, and temporal shifts in the microbial communities were observed in both the
oxic and anoxic layers of Lake Suigetsu during the sampling period. Several dominant DGGE bands were excised and sequenced.
In the chemocline, green sulfur bacteria phylogenetically related to the genera Prosthecochloris, Pelodyctyon, and Chlorobium within the phylum Chlorobi were dominant; the colorless sulfur bacteria closely related to the genus Thiomicrospira were detected. These sulfur bacterial groups appear to be important in the biogeochemical cycling of sulfur and/or carbon
in Lake Suigetsu. Bacterial sequences affiliated with the Bacteroidetes phylum were frequent among the dominant fragments in the DGGE profiles throughout the water column. Populations possessing
a fermentative metabolism exist in Bacteroidetes, suggesting they may contribute to the degradation of organic matter in the anoxic environment of Lake Suigetsu. |
| |
Keywords: | Denaturing gradient gel electrophoresis Meromictic lake Microbial diversity 16S rDNA |
本文献已被 SpringerLink 等数据库收录! |