Actinobacillus pleuropneumoniae metalloprotease: cloning and in vivo expression |
| |
Authors: | García González Octavio García Rosa M de la Garza Mireya Vaca Sergio Paniagua Gloria Luz Mejía Ricardo Tenorio Víctor R Negrete-Abascal Erasmo |
| |
Affiliation: | Carrera de Biología, Facultad de Estudios Superiores Iztacala, UNAM, Av. de los Barrios #1, Los Reyes Iztacala, Tlalnepantla, Estado de México 54090, Mexico. |
| |
Abstract: | The complete amino acid and nucleotide sequence of a secreted metalloprotease produced by Actinobacillus pleuropneumoniae serotype 1 is reported. A clone showing proteolytic activity in cell-free culture media was selected from a genomic library of A. pleuropneumoniae serotype 1 in pUC 19. The sequence obtained contained an open reading frame encoding a protein with 869 amino acids. This protein was identified as a zinc neutral-metalloprotease belonging to the aminopeptidase family, with a predicted molecular weight of approximately 101 kDa. This sequence showed high homology with other predicted or sequenced aminopeptidases reported for different Gram-negative bacteria. Expression of the protease was observed in lung tissue from pigs that died of porcine pleuropneumonia suggesting a role in pathogenesis. |
| |
Keywords: | Protease Pathogenicity Porcine pleuropneumonia Escherichia coli |
本文献已被 PubMed 等数据库收录! |
|