Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA) |
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| Authors: | Mimi Høier-Madsen Steen Ingemann Hansen Jan Holm |
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| Institution: | (1) Laboratory for Autoimmune Serology, State Serum Institute, 2300 S Copenhagen, Denmark;(2) Department of Clinical Chemistry, Central Hospital Hillerød, 3400 Hillerød, Denmark;(3) Department of Clinical Chemistry, Central Hospital Nykøbing Falster, 4800 Nykøbing Falster, Denmark |
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| Abstract: | Antibodies to a low molecular weight folate binding protein isolated from human milk were raised in rabbits and used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for immunological characterization of human folate binding proteins (FBPs). The high and low molecular weight FBPs from human milk were immunologically indistinguishable. Furthermore, the FBPs in human urine and cerebrospinal fluid showed a cross-reactivity of 70% and 30%, respectively. No cross-reactivity of the FBP from cow's milk was observed. |
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| Keywords: | human milk folate binding proteins rabbit antibodies |
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