| 日本血吸虫病患者血清对血吸虫卵和成虫抗原的免疫反应 |
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| 引用本文： | 许阿莲,周晓音,Donald A.Harn. 日本血吸虫病患者血清对血吸虫卵和成虫抗原的免疫反应[J]. 生物化学与生物物理进展, 1992, 19(1): 41-46 |
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| 作者姓名： | 许阿莲 周晓音 Donald A.Harn |
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| 作者单位： | 浙江医科大学寄生虫学教研室;浙江医科大学寄生虫学教研室;美国哈佛大学公共卫生学院热带公共卫生系 |
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| 摘 要： | 用免疫印渍术分析了33例确诊为日本血吸虫病患者血清对日本血吸虫虫卵抗原(SEA)和成虫抗原(SWAP)的免疫反应,发现患者血清对虫卵和成虫抗原的敏感性分别为100％和97％,而正常人血清则无反应,大多数病例(28/33)血清和SEA抗原反应后,印渍谱上于180,54和32kD处显现较宽区带;对SWAP而言,多数病例(26/32)则在190及64kD处显现区带;而急性患者(21/24)还可在34kD处检出一条较宽区带。这些结果提示了免疫印渍术在日本血吸虫病临床诊断上的价值。
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| 关 键 词： | 免疫应答 日本血吸虫病 免疫印渍术 酶联免疫吸附 |
| 收稿时间： | 1990-10-05 |
| 修稿时间： | 1991-01-06 |
IMMUNOREACTIVITY OF PATIENT SERA TO SCHISTOSOME JAPONICUM EGG AND ADULT ANTIGENS |
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| XU Alian,Zhou Xiaoyin and Donald A. Harn. IMMUNOREACTIVITY OF PATIENT SERA TO SCHISTOSOME JAPONICUM EGG AND ADULT ANTIGENS[J]. Progress In Biochemistry and Biophysics, 1992, 19(1): 41-46 |
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| Authors: | XU Alian Zhou Xiaoyin Donald A. Harn |
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| Affiliation: | Department of Parasitology, Zhejiang Medical University;Department of Parasitology, Zhejiang Medical University;Department of Tropical Public Health, Harvard School of Public Health, USA |
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| Abstract: | A sensitive and specific immunoblot assay was used to analyze 33 cases of immune reactions between sera of Schistosomiasis Japonica patients and schistosome Japonicum egg (SEA) and adult (SWAP) antigens. The assays consists of SDS-PAGE and Western blot. The sensitivity of SEA reaction is 100%, while that of SWAP is 97%, with negative reactivity in the control sera. In most cases (28/33), SEA reactive to patient sera show three wide bands at 180,54 and 32kD while in the case of SWAP it shows two wide bands at 190 and 64kD in 26 cases(26/32). In most patients (21/24) with acute schistosomiasis Japonica, an additional wide band is seen at 34kD. These results suggest clinical value of Western Blot in the diagnosis of schistosomiasis Japonica. |
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| Keywords: | immunoreactivity schistosomiasis japonica Western blot ELISA |
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