Differences in amino acid sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from two species of Dasycladaceae |
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Authors: | M B Leible H -G Schweiger |
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Institution: | (1) Max-Planck-Institut für Zellbiologie, D-6802 Ladenburg, Federal Republic of Germany |
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Abstract: | In contrast to other plants the plastid genome of Acetabularia is larger in size and shows a high degree of variability. This study on the chloroplast-encoded large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase demonstrates that strongly conserved areas also exist in the plastid genome of the Dasycladaceae. Searching for differences in the amino acid sequence of the large subunit from Acetabularia mediterranea and Acicularia schenckii, proteolytic peptides which differ in their elution behaviour in reverse-phase high-performance liquid chromatography were sequenced. Only six amino acids were found to be exchanged in the large subunit from these two species. Since these two species diverged approx. 150 million years ago, these results imply that 0.84 amino-acid exchanges per 100 amino acids have occurred in 108 years, underlining the strong conservatism of the large subunit.Abbreviations A
Acetabularia mediterranea
- Ac.
Acicularia schenckii
- HPLC
high-performance liquid chromatography
- LSU
large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase
- PAGE
polyacrylamide gel electrophoresis
- RuBPCase
ribulose-1,5-bisphosphate carboxylase/oxygenase
- SDS
sodium dodecyl sulfate |
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Keywords: | Acetabularia Acicularia Chlorophyta Chloroplast genome Dasycladaceae Evolution Ribulose-1 5-bisphosphate carboxylase (conservatism) |
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