Comparative mapping of the barley genome with male and female recombination-derived, doubled haploid populations |
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| Authors: | Pierre Devaux Andrzej Kilian and Andris Kleinhofs |
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| Institution: | (1) Departments of Crop and Soil Sciences, and Genetics and Cell Biology, Washington State University, 99164-6420 Pullman, WA, USA;(2) Present address: Biotechnology Laboratory, Florimond Desprez Co., P.O. Box 41, 59242 Cappelle-en-Pevele, France;(3) Present address: Department of Genetics, Silesian University, 40-032 Katowice, Poland |
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| Abstract: | Male (anther culture) and female (Hordeum bulbosum) derived, doubled haploid populations were used to map the barley genome and thus determine the different recombination rates occurring during meiosis in the F1 hybrid donor plants. The anther culture-derived (male recombination) population showed an 18% overall increase in recombination rate. This increased recombination rate was observed for every chromosome and most of the chromosome arms. Examination of linkage distances between individual markers revealed eight segments with significantly higher recombination in the anther culture-derived population, and one in the Hordeum bulbosum-derived population. Very strong distortions of single locus segregations were observed in the anther culture-derived population, but map distances were not affected significantly by these distortions. There were 1.047 and 0.912 recombinations per chromosome in the anther culture and Hordeum bulbosum-derived doubled haploid populations, respectively. |
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| Keywords: | Recombination frequency Hordeum vulgare Segregation distortion Selection |
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