The Bacillus stearothermophilus replicative helicase: cloning, overexpression and activity |
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| Authors: | Bird L E Wigley D B |
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| Affiliation: | Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK. |
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| Abstract: | As part of biochemical and structural studies of the primosome of a gram positive bacterial species, we describe the cloning of the Bacillus stearothermophilus replicative helicase, DnaB. The protein is 45% and 82% identical to the Escherichia coli and B. subtilis replicative helicases, respectively. Recombinant DnaB was purified and shown to be an active helicase. |
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