| 测定极端嗜盐古菌保持完整细胞形态最低NaCl浓度研究方法探析 |
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| 引用本文： | 陈绍兴,梁 敏,费宗伟,谢志雄. 测定极端嗜盐古菌保持完整细胞形态最低NaCl浓度研究方法探析[J]. 微生物学通报, 2008, 35(8): 1332-1334 |
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| 作者姓名： | 陈绍兴 梁 敏 费宗伟 谢志雄 |
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| 作者单位： | 1. 红河学院生命科学与技术学院,蒙自,661100
2. 武汉大学生命科学学院,武汉,430072 |
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| 基金项目： | 红河学院硕、博士科研启动基金 |
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| 摘 要： | 细菌要维持其完整的细胞形态需要一定的渗透压,根据极端环境微生物耐盐程度的差异大致分为非嗜盐、弱嗜盐、中等嗜盐、极端嗜盐和耐盐菌5大类.本研究组通过测定不同浓度NaCl条件处理下,600 nm处的光谱吸收的改变值结合显微照相的方法,对极端嗜盐古菌维持完整细胞形态所必须的最低NaCl浓度进行研究.发现极端嗜盐古菌CY1保持完整的细胞形态的最低NaCl浓度为8%～10%.并建立了较为系统、可靠的测定极端嗜盐古菌保持完整细胞形态最低NaCl浓度的研究方法.
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| 关 键 词： | 极端嗜盐古菌 耐盐程度 细胞形态 |
Methods on Detecting the Minimum NaCl Concentration Which Could Maintain the Integrity Cell Morphology from Extremely Halophilic Archaea |
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| CHEN Shao-Xing,LIANG Min,FEI Zong-Wei and XIE Zhi-Xiong. Methods on Detecting the Minimum NaCl Concentration Which Could Maintain the Integrity Cell Morphology from Extremely Halophilic Archaea[J]. Microbiology China, 2008, 35(8): 1332-1334 |
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| Authors: | CHEN Shao-Xing LIANG Min FEI Zong-Wei XIE Zhi-Xiong |
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| Affiliation: | College of Life Sciences, Honghe University, Mengzi 661100;College of Life Sciences, Honghe University, Mengzi 661100;College of Life Sciences, Honghe University, Mengzi 661100;College of Life Sciences, Wuhan University, Wuhan 430072 |
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| Abstract: | Bacteria had to been in a certain degree of osmolality to maintenance intact cell figure. This study carried on the research on lowest concentration of NaCl which to keep complete cells figure of extremely halophilic archaea strain CY1. The research methods were measuring the change of optical density at 600 nm by dealing with different concentration of NaCl. Microscope figures were taken after a simple staining. The lowest concentration of NaCl was 8% ~10%. |
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| Keywords: | Extremely halophlic archaea Salt tolerance Cell morphology |
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