| 大肠癌细胞GST-Pi免疫细胞化学的超微结构定位 |
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| 引用本文： | 郭文君,刘雨清,吴洪娟. 大肠癌细胞GST-Pi免疫细胞化学的超微结构定位[J]. 中国组织化学与细胞化学杂志, 2000, 9(3): 319-321 |
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| 作者姓名： | 郭文君 刘雨清 吴洪娟 |
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| 作者单位： | 潍坊医学院病理学教研室，山东261042 |
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| 基金项目： | 山东省卫生厅资助课题!(No. 1998118) |
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| 摘 要： | 探讨GST－Pi在大肠癌细胞内亚微结构定位及其与大肠癌发生的关系。应用免疫电镜技术（免疫胶体金法）对6例大肠腺癌及3例正常大肠粘膜细胞进行GST－Pi的定位观察。6例大肠癌细胞内均出现GST－Pi胶体金阳性颗粒，主要分布于胞浆的线粒体、溶酶体、近胞膜部位的胞头中，也分布在核内和核膜上。金颗粒呈团状、点灶状分布，图象清晰。3例正常粘膜细胞内未见GST－Pi的金颗粒阳性表达，结果表明，GST－Pi在癌细胞内的特异性表达可作为大肠癌的诊断指标之一。
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| 关 键 词： | 大肠癌 GST－Pi 免疫电镜 胶体金 癌细胞 免疫细胞化学 超微结构定位 |
ULTRASTRUCTURAL LOCALIZATION OF GLUTATHIONE S-TRANSFERASE-PI IN HUMAN COLORECTAL CANCER CELLS |
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| Guo Wenjun,Liu Yuqing,Wu Hongjuan,et al. ULTRASTRUCTURAL LOCALIZATION OF GLUTATHIONE S-TRANSFERASE-PI IN HUMAN COLORECTAL CANCER CELLS[J]. Chinese Journal of Histochemistry and Cytochemistry, 2000, 9(3): 319-321 |
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| Authors: | Guo Wenjun Liu Yuqing Wu Hongjuan et al |
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| Abstract: | The purpose of ourstudy is to explore the significance of the ultrastructural localization of glutathione S- transferase- pi ( GST- Pi) in human colorectal cancer cells and the relationship between colorectal carcinogensis and GST- Pi.Colloid gold labelling and immunoelectron microscope technique were used for the localization of GST- Pi in3cases of normal colorectal mucosa and6cases of colorectal adenocarcinoma. The result showed that GST- Pi colloid gold particles in colorectal cancer cells were localized in mitochondria,lysosome,nucleus and nucleus membrane.GST- Picolloid gold particles were distributed in spotty group. GST- Pi was expressed negatively in 3cases of normal colorectal mucosa. The findings suggest that GST- Pi special expression in colorectal cancer cells may be used as a marker to diagnose colorectal carcinoma. |
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| Keywords: | Colorectal cancer GST- Pi Immunoelectron microscope Colloid gold Human |
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