Enhancing reaction rate for transesterification reaction catalyzed by Chromobacterium lipase |
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Authors: | Ipsita Roy Munishwar N Gupta |
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Institution: | Department of Chemistry, Indian Institute of Technology, Hauz Khas, New Delhi 110016, India |
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Abstract: | Precipitation of Chromobacterium viscosum lipase as an interfacial layer by addition of ammonium sulphate and t-butanol in a process called three phase partitioning (TPP)] led to an increase in the initial rate of tranesterification in iso-octane by 4.9 times. It is shown that the TPP-treated lipase also showed salt activation and further increase in activity in the presence of sorbitol. Thus, in synergy with these strategies, the TPP treatment resulted in a more efficient design of catalytic transesterification; the overall increase in transesterification as a result of the optimization was about 15 times. |
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Keywords: | Enzymes in organic media Lipase Three phase partitioning Transesterification reactions |
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