| 双歧杆菌发酵果蔬汁中低聚果糖的高效液相色谱法分析 |
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| 引用本文： | 龚芳红,贺松,张德纯,郭亚楠,王永红.双歧杆菌发酵果蔬汁中低聚果糖的高效液相色谱法分析[J].中国微生态学杂志,2010,22(6):489-491. |
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| 作者姓名： | 龚芳红 贺松 张德纯 郭亚楠 王永红 |
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| 作者单位： | 1. 重庆医科大学,分子医学与肿瘤研究中心,病原生物学教研室,重庆,400016
2. 重庆市动物疫病预防控制中心,重庆,401120 |
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| 基金项目： | 重庆市科委十一五重点科技公关项目 |
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| 摘 要： | 目的采用高效液相色谱法（HPLC）对双歧杆菌发酵果蔬汁中的低聚果糖进行分析。方法 HPLC分析条件为：ZORBAX NH2 Analytical色谱柱,乙腈∶水（75∶25）为流动相,柱温35℃,流速为1.0ml/min,示差折光检测器（RID）。结果果蔬汁中的低聚果糖及几种主要糖类均得到有效分离。此分离方法的加标回收率和精密度（RSD）均较高。结论分析结果表明双歧杆菌发酵果蔬汁中含有较丰富的功能性低聚果糖,尤其是蔗果三糖含量很高。
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| 关 键 词： | 双歧杆菌发酵果蔬汁 低聚果糖 高效液相色谱法 |
Analysis of fructo-oligosaccharides in Bifidobacterium-fermented mixed fruit and vegetable juice by high performance liquid chromatography |
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| GONG Fang-hong,HE Song,ZHANG De-chun,GUO Ya-nan,WANG Yong-hong.Analysis of fructo-oligosaccharides in Bifidobacterium-fermented mixed fruit and vegetable juice by high performance liquid chromatography[J].Chinese Journal of Microecology,2010,22(6):489-491. |
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| Authors: | GONG Fang-hong HE Song ZHANG De-chun GUO Ya-nan WANG Yong-hong |
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| Institution: | GONG Fang-hong1,HE Song1,ZHANG De-chun1,GUO Ya-nan1,WANG Yong-hong2( 1.Department of Pathogenic Biology,Molecular Medicine & Tumor Research Center,Chongqing Medical University,Chongqing 400016,China,2.Center for Animal Disease Control and Prevention of Chonqing,Chongqing,401120,China) |
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| Abstract: | Objective To investigate the fructo-oligosaccharides (FOS) content in Bifidobacterium-fermented mixed fruit and vegetable juice by high performance liquid chromatography.Method A ZORBAX NH2 analytical column was used with acetonitrile-water solution (75:25,v/v) as the mobile phase at a flowrate of 1.0 ml/min,and a refractive index detector(RID)was used for the detection of the sugars.Result Under the above conditions,the main sugars in Bifidobacterium-fermented mixed fruit and vegetable juice had been effec... |
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| Keywords: | Bifidobacterium-fermented mixed fruit and vegetable juice Fructo-oligosaccharides HPLC |
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