复苏植物旋蒴苣苔棉子糖合酶基因的克隆和表达

复苏植物是研究植物耐脱水机制的特殊模式植物和宝贵的耐旱基因资源植物。以复苏植物旋蒴苣苔(Boea hygrometrica) 为材料研究其在脱水和复水过程中棉子糖系列寡糖含量的变化, 并克隆了旋蒴苣苔棉子糖合酶基因BhRFS。荧光定量PCR检测表明, BhRFS受干旱、低温(4°C)、高盐(200 mmol·L^–1NaCl)和ABA(100 μmol·L^–1)诱导表达上调, 而高温(37°C)抑制其表达, H₂O₂(200 μmol·L^–1)处理对其没有影响。研究结果表明, BhRFS可能参与了多种非生物逆境胁迫抗性反应, 并受到ABA依赖的信号通路调控。

Cloning and Expression of a Gene Encoding a Raffinose Synthase in the Resurrection Plant Boea hygrometrica

Resurrection plants are a valuable resource for understanding the mechanisms of dehydration tolerance and for mining candidate genes that can be used to improve drought tolerance in crops. Here, we determined the contents of galactinol, raffinose and stachyose in dehydrated and rehydrated leaves of a resurrection plant Boea hygrometrica. We cloned a dehydration-inducible gene encoding a raffinose synthase from B. hygrometrica and found that BhRFS was upregulated by cold (4°C), high salt (200 mmol·L^–1NaCl), and abscisic acid (ABA, 100 μmol·L^–1) but downregulated by high temperature (37°C) and not affected by H₂O₂ (200 μmol·L^–1). BhRFS may function in responce to various abiotic stresses, possibly regulated by ABA-dependent signal pathways, therefore it is a good candidate gene for plant breeding to enhance plant stress tolerance.