| 烟酸对HepG2细胞载脂蛋白M表达的影响及其机制 |
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| 引用本文： | 杨柳,赵水平.烟酸对HepG2细胞载脂蛋白M表达的影响及其机制[J].现代生物医学进展,2010,10(2). |
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| 作者姓名： | 杨柳 赵水平 |
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| 作者单位： | 中南大学湘雅二医院心血管内科,湖南,长沙,410011 |
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| 摘 要： | 目的:探讨烟酸对HepG2细胞载脂蛋白M表达的影响及其机制。方法:分别以不同浓度的烟酸(0、0.25、0.5、1.0、2.0mmol/L)干预HepG2细胞24h。提取各组细胞总RNA和蛋白质,分别采用实时R T-PCR和Western Blot检测载脂蛋白M的mRNA和蛋白的表达。采用实时R T-PCR检测肝细胞核因子-1αmRNA的表达。结果:烟酸呈剂量依赖性上调载脂蛋白M基因和蛋白、肝细胞核因子-1α基因的表达(P<0.05)。结论:烟酸可上调载脂蛋白M表达,其机制可能是通过上调肝细胞核因子-1α来实现的。
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| 关 键 词： | 烟酸 载脂蛋白M 肝细胞核因子-1α |
Effects and Mechanism of Niacin on the Expression of Apolipoprotein M |
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| YANG Liu,ZHAO Shui-ping.Effects and Mechanism of Niacin on the Expression of Apolipoprotein M[J].Progress in Modern Biomedicine,2010,10(2). |
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| Authors: | YANG Liu ZHAO Shui-ping |
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| Institution: | YANG Liu,ZHAO Shui-ping(Department of Cardiology,the Second Xiang ya Hospital of Central South University,Changsha 410011,China) |
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| Abstract: | Objective:To examine the effects and mechanism of niacin on the expression of apolipoprotein M in HepG2 cells. Methods:The HepG2 cells were incubated with different concentration of niacin( 0,0.25,0.5,1.0,2.0mmol /L) for 24h. Total RNA and protein of HepG2 cells were extracted. The levels of apoMmRNA and protein were measured by real time RT-PCR and Western blot. The expression of hepatic nuclear factor(HNF) -1amRNA was measured by real time RT-PCR. Results:Niacin can dose-dependently increase the expressio... |
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| Keywords: | Niacin Apolipoprotein M Hepatic nuclear factor-1a |
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