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Inhibition of coaggregation between Porphyromonas gingivalis and Streptococcus oralis by fibrinogen fragments |
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| Authors: | Hideki Nagata Atsuo Amano Takashi Hanioka Hiroo Tamagawa Satoshi Shizukuishi Toshiyuki Miyata |
| Affiliation: | Unitéde Mycologie, Institut Pasteur, Paris, France; Service de Dermatologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland; Genethon, Evry, France; Department of Medicine, Division of Allergy and Clinical Immunology, University of Virginia, Charlottesville, Virginia, USA; TNO Medical Biological Laboratory, Rijswijk, the Netherlands |
| Abstract: | Abstract The aspfI gene encoding a ribonucleotoxin, a putative virulence factor of Aspergillus fumigatus , was inactivated by gene disruption. Gene replacement through homologous recombination by the disrupted allele tagged by the hygromycin B resistance marker was performed by transformation of a pathogenic strain. One transformant with the disrupted aspfI gene failed to produce the ASPFI protein and was shown to be pathogenic for mice. We concluded that this ribotoxin is not a main factor in the colonization of the lung tissues by A. fumigatus . |
| Keywords: | Aspergillus fumigatus Restrictocin Gene disruption Allergen ASPFI protein Antigen Pathogenicity |