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In silico and experimental improvement of bacteriorhodopsin production in Halobacterium salinarum R1 by increasing DNA-binding affinity of Bat through Q661R/Q665R substitutions in HTH motif
Authors:Mirfeizollahi  Azadeh  Yakhchali  Bagher  Deldar  Ali Asghar  Karkhane   Ali Asghar
Affiliation:1.Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Km 15, Tehran-Karaj Highway, P.O. Box 14965/161, Tehran, Iran
;2.Department of Bioscience, Institute of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
;
Abstract:Extremophiles - DNA-binding motif of bacterioopsin activator (Bat) protein is a Helix–Turn–Helix motif, which binds to bop promoter and induces bacterioopsin (Bop) expression under...
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