Murine monocytes express transferrin receptors: evidence for similarity to inflammatory macrophages |
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Authors: | J E Weiel D O Adams T A Hamilton |
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Affiliation: | 1. Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710 U.S.A.;2. Department of Microbiology-Immunology, Duke University Medical Center, Durham, North Carolina 27710 U.S.A. |
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Abstract: | Nonionic detergent extracts of murine monocytes contain a specific, high-affinity binding site for the serum glycoprotein transferrin. The binding activity was saturable and specific for 125I-labeled transferrin. The transferrin-receptor content of monocytes was compared with that of resident peritoneal macrophages and thioglycollate-elicited macrophages. Whereas resident cells showed no detectable activity, inflammatory macrophages and monocytes both bound transferrin to a similar degree. The calculated dissociation constant (2.3 X 10(-10)) and the number of sites per monocyte (11,400) compared favorably with those reported for transferrin receptors on inflammatory macrophages. Thus, based on the expression of the transferrin receptor, murine monocytes resemble murine inflammatory peritoneal macrophages rather than resident tissue macrophages. |
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Keywords: | To whom correspondence should be addressed at: Department of Pathology Box 3712 Duke University Medical Center Durham N.C. 27710. |
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