Hydrolysis of Butteroil by Immobilized Lipase Using a Hollow-Fiber Reactor: Part VI. Multiresponse Analyses of Temperature and pH Effects |
| |
Authors: | F. Xavier Malcata Charles G. Hill Jr. Clyde H. Amundson |
| |
Affiliation: | a Department of Chemical Engineering, University of Wisconsin, Madison, WI, USAb Escola Superior de Biotecnologia, Universidade Catolica Portuguesa, Rua Dr. Antonio Bernardino de Almeida, Porto, Portugalc Department of Food Science, University of Wisconsin, Madison, WI, USAd Department of Agricultural Engineering, University of Wisconsin, Madison, WI, USA |
| |
Abstract: | A lipase from Aspergillus niger, immobilized by physical adsorption on hydrophobic hollow fibers made of microporous polypropylene, was used to effect the hydrolysis of the glycerides of melted butterfat at 40, 50, 55, and 60°C (pH 7.0), and at pH 3.0, 4.0, 5.0, 7.0, 8.0, and 9.0 (40°C). McIlvane buffer and melted butterfat were pumped cocurrently through the hollow fiber reactor. The concentrations of ten different free fatty acids in the effluent oil stream were measured by HPLC. Multiresponse nonlinear regression methods were employed to fit the data to multisubstrate rate expressions derived from a Ping Pong Bi Bi mechanism in which the rate controlling step is deacylation of the enzyme. Thermal deactivation of the immobilized lipase was also included in the mathematical model of reactor performance. A postulated normal distribution of vmax with respect to the number of carbon atoms of the fatty acid residue (with an additive correction for the number of double bonds) was found to provide the best statistical fit of the data. The models developed can be used to independently predict the effects of either the pH or the temperature, as well as the reactor space time and the time elapsed after immobilization, on the free fatty acid profile of the lipolyzed butteroil product. |
| |
Keywords: | Immobilized lipase Hollow fiber reactor Lipolysis Butteroil Butterfat Effects of pH Effects of temperature Multiresponse analysis |
本文献已被 InformaWorld 等数据库收录! |
|